The Sharma lab focuses on understanding the role of splicing factor mutations in the pathogenesis of myeloid malignancies, including acute myeloid leukemia, myelodysplastic syndrome and chronic myelomonocytic leukemia. The goal is to determine the impact of splicing factor mutations on proliferation and myeloid differentiation of human hematopoietic stem cells, as well as to identify mutation-induced aberrations in pre-RNA splicing and protein expression that can be targeted for therapy.