Splicing refers to a process in molecular biology where a pre-mRNA molecule is edited or modified to remove introns and connect exons before the mature mRNA is translated into a protein. This process is essential for gene expression and plays a crucial role in regulating gene function and diversity. Errors in splicing can lead to genetic diseases and have been implicated in various human disorders. Various mechanisms and factors are involved in splicing, including spliceosomes, splice sites, and regulatory proteins. Studying splicing can provide insights into the complexity of gene expression and contribute to understanding genetic diseases and therapeutics.