Rhamnolipids are a type of biosurfactant produced by various species of bacteria, particularly Pseudomonas aeruginosa. They have surface-active properties, which make them effective in reducing the surface tension of liquids and aiding in the emulsification of hydrophobic compounds. Rhamnolipids have a wide range of applications in industries such as bioremediation, agriculture, and pharmaceuticals, due to their biodegradability and low toxicity. Research in this area focuses on understanding the production mechanisms of rhamnolipids, optimizing their production, and exploring their potential applications in various fields.