Rhamnolipids are a type of biosurfactant produced by various species of bacteria, particularly Pseudomonas aeruginosa. These molecules are composed of a rhamnose sugar molecule linked to one or two hydroxy fatty acids, and they have both hydrophilic and hydrophobic properties which allow them to reduce the surface tension of liquids and form micelles. Rhamnolipids have shown great potential in various applications such as enhanced oil recovery, bioremediation, pharmaceuticals, cosmetics, and food industry due to their biodegradability, low toxicity, and high surface activity. Research in this area focuses on improving the production yield, understanding the mechanisms of rhamnolipid biosynthesis, exploring new applications, and developing cost-effective and sustainable production methods.