Autofluorescence is the natural fluorescence emitted by certain molecules within cells and tissues when exposed to light. This phenomenon is commonly used in various research areas, such as fluorescence microscopy, flow cytometry, and biomedical imaging, to study the distribution and characteristics of specific molecules or structures within biological samples. Autofluorescence can provide information about the composition and metabolism of cells, as well as the presence of certain biomolecules or diseases. By detecting autofluorescence, researchers can gain insights into the physiological and pathological processes occurring within living organisms.